The analytical performance characteristics of this test have been determined by Quest Diagnostics Nichols Institute, San Juan Capistrano. The mean level of glycocalicin in these patients was decreased compared to that in controls and patients with AITP, but was not as low as in patients with amegakaryocytic thrombocytopenia. Fourteen children with congenital thrombocytopenia were analysed in order to unravel the mechanisms underlying their thrombocytopenia and to evaluate the value of new laboratory tests, namely measurement of plasma thrombopoietin (Tpo) and glycocalicin (GC) levels and analysis of megakaryocytopoiesis in vitro. Thrombopoietin (TPO) - This test will be used to measure serum TPO level in patients with thrombocytopenia of unknown cause. The analytical performance characteristics of this test have been determined by Quest Diagnostics Nichols Institute, San Juan Capistrano. Each patient underwent physical examination and routine laboratory tests, and was prospectively followed for >6 months. To diagnose immune thrombocytopenia, your doctor will try to exclude other possible causes of bleeding and a low platelet count, such as an underlying illness or medications you or your child may be taking.Blood tests can check the levels of platelets. The analytical performance characteristics of this test have been determined by Quest Diagnostics Nichols Institute, San Juan Capistrano. It is used to diagnose megakaryocytopenia and thrombocytopenia, among other blood cell deficiencies The MPL Mutation Analysis Test also aids in the treatment of blood cell disorders by guiding selection of therapeutic drugs, including disqualifying certain drugs from use The MPL Mutation Analysis Test is a genetic test that detects abnormalities in the MPL gene. TPO levels were determined in plasma from 178 clinically and serologically well-defined thrombocytopenic patients: 72 patients with idiopathic autoimmune thrombocytopenia (AITP), 29 patients with secondary AITP, 5 patients with amegakaryocytic thrombocytopenia and 72 patients who suffered from various diseases (46 in whom megakaryocyte deficiency was not and 26 in whom it was expected). 2013 Jul;98(1):24-33. doi: 10.1007/s12185-013-1370-4. Synonyms. All Rights Reserved. This test was performed using a kit that has not been approved or cleared by the FDA. Please enable it to take advantage of the complete set of features! 2001 May;115(5):656-64. doi: 10.1309/RAW2-0LQW-8YTX-941V. Careers. 500 Chipeta Way Salt Lake City, UT 84108. 1-800-522-2787 Email 8600 Rockville Pike This assay has a sensitivity to detect approximately 10% population of cells containing the MPL mutations in a background of nonmutant cells. Thrombopoietin (THPO) also known as megakaryocyte growth and development factor (MGDF) is a protein that in humans is encoded by the THPO gene. Deficiencies of the naturally occurring anticoagulants antithrombin, protein C and protein S have been linked with familial venous thrombosis. Click here for your pricing. Serum thrombopoietin (TPO) levels in patients with amegakaryocytic thrombocytopenia are much higher than those with immune thrombocytopenic purpura. Porcelijn L, Schmidt DE, van der Schoot CE, Vidarsson G, de Haas M, Kapur R. Haematologica. Kuter DJ, Begley CG. Plain Red. If the bone marrow is normal but the spleen is enlarged, increased splenic sequestration is the likely cause of thrombocytopenia. Epub 2019 Jul 11. These tests are not needed for diagnosis. doi: 10.3324/haematol.2019.228908. Indicates test has been approved by the New York State Department of Health. Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 month. May include related or preferred tests. Similarly, among the 72 thrombocytopenic patients with various disorders, elevated TPO levels were only found in patients in whom platelet production was depressed. The codes reflect our interpretation of CPT coding requirements based upon AMA guidelines published annually. ITP (Idiopathic Thrombocytopenia purpura) is a common cause of thrombocytopenia. A healthy person usually has a platelet count of 150,000 to 400,000. GTR Test ID Help Each Test is a specific, orderable test from a particular laboratory, and is assigned a unique GTR accession number. CPT Code(s) 83520. Transfer 1 mL serum to an ARUP Standard Transport Tube. FFTPO: Thrombopoietin (TPO) 43237-7 Int J Hematol. [3] The diagnosis of this type of pseudothrombocytopenia is established if the platelet count is normal when repeated on a sample fro… Thrombopoietin, primarily produced in the liver, stimulates the bone marrow to make large cells (megakaryocytes), which in turn make platelets from their cytoplasm. May be useful in certain research settings, Do not use for routine evaluation of platelet disorders. With our TPO ELISA and a glycocalicin ELISA we analysed a large group of patients in detail and could confirm and amplify the above notion in detail. These tests are not needed for diagnosis. A PTT test looks at one set of these factors to see how well they’re working. Thrombocytopenia should always be confirmed by examination of a peripheral smear. Test Classification This test was performed using a kit that has not been approved or cleared by the FDA. Methodology. 1988 Jun; 8(2):303-323 (review). 2020 May;18(5):1210-1220. doi: 10.1111/jth.14762. CPT Codes The American Medical Association Current Procedural Terminology (CPT) codes published in ARUP's Laboratory Test Directory are provided for informational purposes only. In conclusion, all patients with depressed platelet production had elevated levels of circulating TPO, whereas the TPO levels in patients with an immune-mediated thrombocytopenia were mostly within the normal range. Thrombopoietin (THPO) also known as megakaryocyte growth and development factor (MGDF) is a protein that in humans is encoded by the THPO gene.. Thrombopoietin is a glycoprotein hormone produced by the liver and kidney which regulates the production of platelets.It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Anti-GPIIb/IIIa antibody-producing B cells, platelet-associated anti-GPIIb/IIIa antibodies, reticulated platelets and plasma thrombopoietin were also examined at first visit. Recent advances in laboratory tests for autoantibodies to thyrotropin receptor protein in Graves' disease. In addition, we measured the level of glycocalicin as a marker of total body mass of platelets. The workup for immune thrombocytopenic purpura (ITP) starts with a complete blood cell (CBC) count. Other names that describe the test. Immune thrombocytopenia is suspected in patients with isolated thrombocytopenia (ie, otherwise normal CBC and peripheral blood smear). Plasma thrombopoietin levels were measured in 120 patients who had myelodysplastic syndrome with thrombocytopenia (< 100 × 10 … May also include abnormal ranges. For questions regarding the Interface Map, please contact interface.support@aruplab.com. It has been reported that blood trombopoietin (TPO) levels can discriminate between thrombocytopenia due to increased platelet destruction and decreased platelet production. Recommendations when to order or not order the test. Heritable thrombophilia describes an inherited tendency to develop venous thrombosis (DVT and/or PE)1. Immune thrombocytopenic purpura (ITP), also known as idiopathic thrombocytopenic purpura or immune thrombocytopenia, is a type of thrombocytopenic purpura defined as an isolated low platelet count with a normal bone marrow in the absence of other causes of low platelets. 3284698 Schmidt DE, Heitink-Polle KMJ, Porcelijn L, van der Schoot CE, Vidarsson G, Bruin MCA, de Haas M. J Thromb Haemost. Am J Clin Pathol. Fatih Ozcelik, Umran Keskin, Emrah Kılıçaslan, Ebru Kale, Laboratory Tests Used in the Diagnosis of Immune Thrombocytopenia and General Treatment Approaches, Journal of Hematology and Oncology Research, 10.14302/issn.2372-6601.jhor-20-3372, 3, 4, (22-36), (2020). Molecular-based testing is highly accurate, but, as in any laboratory test, rare diagnostic errors may occur. The clot stops you from losing too much blood. This assay will not detect the mutation below the sensitivity of this assay. Background information for test. Expected turnaround time for a result, beginning when ARUP has received the specimen. Normal range/expected value(s) for a specific disease state. Normally, when you get a cut or injury that causes bleeding, proteins in your blood called coagulation factors work together to form a blood clot. This assay has a sensitivity to detect approximately 10% population of cells containing the MPL mutations in a background of nonmutant cells. Anti-glycoprotein Ibα autoantibodies do not impair circulating thrombopoietin levels in immune thrombocytopenia patients. The analytical performance characteristics of this test have been determined by Quest Diagnostics Nichols Institute, San Juan Capistrano. The MPL gene provides instructions for making the thrombopoietin receptor protein, which promotes the growth and division (proliferation) of cells. The potential role of thrombopoietin in idiopathic thrombocytopenic purpura. This receptor is especially important for the proliferation of certain blood cells called megakaryocytes, which produce platelets, the cells involved in blood clotting. You have thrombocytopenia if your number falls under 150,000. 2007 Oct;44(4 Suppl 5):S3-S11. Molecular-based testing is highly accurate, but, as in any laboratory test, rare diagnostic errors may occur. Although myelodysplastic syndromes are heterogeneous disorders comprising a benign subset of bone marrow failure similar to aplastic anemia, no laboratory test has been established to distinguish it from bone marrow failures that can evolve into acute myeloid leukemia. Thrombocytopenia is defined as a platelet count below the 2.5th lower percentile of the normal platelet count distribution. What are other Names for this Test? Novel thrombopoietic agents: a review of their use in idiopathic thrombocytopenic purpura. Fatih Ozcelik, Umran Keskin, Emrah Kılıçaslan, Ebru Kale, Laboratory Tests Used in the Diagnosis of Immune Thrombocytopenia and General Treatment Approaches, Journal of Hematology and Oncology Research, 10.14302/issn.2372-6601.jhor-20-3372, 3, 4, (22-36), (2020). Immunoassay. The American Medical Association Current Procedural Terminology (CPT) codes published in ARUP's Laboratory Test Directory are provided for informational purposes only. These values do not apply to results that are converted to other units of measure. May include disease information, patient result explanation, recommendations, details of testing, associated diseases, explanation of possible patient results. Epub 2020 Apr 12. Platelet Function Tests Platelets (sometimes called thrombocytes) are cell fragments that circulate in the bloodstream and help blood to clot . Test Result Name Result LOINC Value Applies only to results expressed in units of measure originally reported by the performing laboratory. 2020 Apr;105(4):e172-e174. This test should not be used for diagnosis without confirmation by other medically established means. Three groups of patients were included. It causes a characteristic red or purple bruise-like rash and an increased tendency to bleed. The hallmark of ITP is isolated thrombocytopenia; anemia and/or neutropenia may indicate other diseases Clumps of platelets on a peripheral smear prepared from ethylenediaminetetraacetic acid (EDTA)anticoagulated blood are evidence of pseudothrombocytopenia. With normal platelet function, thrombocytopenia is rarely the cause of bleeding unless the count is less than 50,000/mm 3. You have several coagulation factors in your blood. Thrombocytopenia is defined as a platelet count of less than 150,000/mm 3. Clin Lab Med. 115: 2001; 656-664. In all patients with primary AITP and secondary AITP, TPO levels were within the normal range or in some (n = 7) cases only slightly increased. These tests are not needed for diagnosis. Anti-platelet antibodies in childhood immune thrombocytopenia: Prevalence and prognostic implications. Clinical Significance. It is used to diagnose megakaryocytopenia and thrombocytopenia, among other blood cell deficiencies. It has been reported that blood trombopoietin (TPO) levels can discriminate between thrombocytopenia due to increased platelet destruction and decreased platelet production. Although the concept of a platelet growth factor analogous to erythropoietin had been proposed in the 1950s, it was not until 1994 that the existence of this hematopoietic … Accessibility 2017 May;8(5):159-174. doi: 10.1177/2040620717693573. von dem Borne A, Folman C, van den Oudenrijn S, Linthorst G, de Jong S, de Haas M. Blood Rev. Prevention and treatment information (HHS). Reference Range(s) 7-99 pg/mL. Nonspecific laboratory abnormalities that may occur in polycythemia vera include elevated vitamin B12 and B12-binding capacity, hyperuricemia and hyperuricosuria (present in ≥ 80% of patients), and decreased expression of MPL (the receptor for thrombopoietin) in megakaryocytes and platelets. Therefore, measurement of plasma TPO levels provides valuable diagnostic information for the analysis of thrombocytopenia in general. Would you like email updates of new search results? A PTT test looks at one set of these factors to see how well they’re working. National Library of Medicine Drugs. MPL Exon 10 Mutation Analysis. Nonspecific laboratory abnormalities that may occur in polycythemia vera include elevated vitamin B12 and B12-binding capacity, hyperuricemia and hyperuricosuria (present in ≥ 80% of patients), and decreased expression of MPL (the receptor for thrombopoietin) in megakaryocytes and platelets. This test was performed using a kit that has not been approved or cleared by the FDA. Thrombopoietin (TPO) is the physiologically relevant regulator of platelet production. Kurata Y, Hayashi S, Kiyoi T, Kosugi S, Kashiwagi H, Honda S, Tomiyama Y. doi: 10.1053/j.seminhematol.2007.11.002. The factor V Leiden (1691G>A, R506Q) and prothrombin (20210G>A) gene variants are associated with an increased risk of venous thrombosis (see Table 1 for relative risks). Nonspecific laboratory abnormalities that may occur in polycythemia vera include elevated vitamin B12 and B12-binding capacity, hyperuricemia and hyperuricosuria (present in ≥ 80% of patients), and decreased expression of MPL (the receptor for thrombopoietin) in megakaryocytes and platelets. Web: mayocliniclabs.com: Email: mcl@mayo.edu: Telephone: 800-533-1710: International: +1 855-379-3115: Values are valid only on day of printing. A partial thromboplastin time (PTT) test measures the time it takes for a blood clot to form. The format is GTR00000001.1, with a leading prefix 'GTR' followed by 8 digits, a period, then 1 or more digits representing the version. Unable to load your collection due to an error, Unable to load your delegates due to an error. Quest Diagnostics San Juan Capistrano Inc. © 2021 ARUP Laboratories. From chronic immune thrombocytopenia to severe aplastic anemia: recent insights into the evolution of eltrombopag. Anti-GPIIb/IIIa antibody-producing B cells, platelet-associated anti-GPIIb/IIIa antibodies, reticulated platelets and plasma thrombopoietin were also examined at first visit. 2002 Mar;16(1):57-9. doi: 10.1054/blre.2001.0184. Qty: Description ... Reference Range(s) 7-99 pg/mL. FOIA The pathogenesis of chronic immune thrombocytopenic purpura. Clipboard, Search History, and several other advanced features are temporarily unavailable. Plasma thrombopoietin levels were measured in 120 patients who had myelodysplastic syndrome with thrombocytopenia (< 100 × 10 (9)/L) to determine any correlation to markers associated with immune pathophysiology and outcome. Thrombopoietin (TPO) - This test will be used to measure serum TPO level in patients with thrombocytopenia of unknown cause. The codes reflect our interpretation of CPT coding requirements based upon AMA guidelines published annually. The level of glycocalicin was not significantly different from that of the controls (n = 95). Bone marrow aspiration reveals the number and appearance of megakaryocytes and is the definitive test for many disorders that cause bone marrow failure. With our TPO ELISA and a glycocalicin ELISA we analysed a large group of patients in detail and could confirm and amplify the above notion in detail. Bethesda, MD 20894, Copyright Rarely, adults might need a bone marrow exam to rule out other problems. Diagnostic value of tests for reticulated platelets, plasma glycocalicin, and thrombopoietin levels for discriminating between hyperdestructive and hypoplastic thrombocytopenia. Each patient underwent physical examination and routine laboratory tests, and was prospectively followed for >6 months. Get the Lowest Prices and Visit your Local Lab. This test should not be used for diagnosis without confirmation by other medically established means. No Doctor's Visit Needed ... Save On Labs > Test Menu > THROMBOPOIETIN (TPO) Our Price: $ 81.50. Mukai HY, Kojima H, Todokoro K, Tahara T, Kato T, Hasegawa Y, Kobayashi T, Ninomiya H, Nagasawa T, Abe T. Koike Y, Yoneyama A, Shirai J, Ishida T, Shoda E, Miyazaki K, Sunaga S, Horie R, Aoki K, Koike K, Ogata I, Tahara T, Kato T, Nakahara K, Kariya T, Higashihara M. Semin Hematol. FFTPO: Thrombopoietin (TPO) 43237-7 Also acceptable: Serum Separator Tube (SST). Pathophysiology and management of primary immune thrombocytopenia. This test should not be used for diagnosis without confirmation by other medically established means. Additional information related to the test. This site needs JavaScript to work properly. Test Result Name Result LOINC Value Applies only to results expressed in units of measure originally reported by the performing laboratory. Kurata Y, Hayashi S , Kiyoi T. Diagnostic value of tests for reticulated platelets, plasma glycocalicin, and thrombopoietin levels for discriminating between hyperdestructive and hypoplastic thrombocytopenia. Evaluation of thrombopoiesis in thrombocytopenic disorders by simultaneous measurement of reticulated platelets of whole blood and serum thrombopoietin concentrations. This test will be used to measure serum TPO level in patients with thrombocytopenia of unknown cause. ITP (Idiopathic Thrombocytopenia purpura) is a common cause of thrombocytopenia. The patients with amegakaryocytic thrombocytopenia had strongly elevated TPO levels and significantly decreased glycocalicin levels. COVID-19 is an emerging, rapidly evolving situation. This test should not be used for diagnosis without confirmation by other medically established means. This test will be used to measure serum TPO level in patients with thrombocytopenia of unknown cause. Epub 2017 Feb 1. Clinical Significance. Results of the third US National Health and Nutrition Examination Survey (NHANES III) support the traditional value of 150 × 10 9 /L as the lower limit of normal. Are you an ARUP Client? Test Classification This test was performed using a kit that has not been approved or cleared by the FDA. Moreover, treatment with TPO may be an option in AITP. These values do not apply to results that are converted to other units of measure. Am J Clin Pathol. Epub 2013 May 24. 2008;68(7):901-12. doi: 10.2165/00003495-200868070-00002. Ther Adv Hematol. Privacy, Help (Min: 0.5 mL)Test is not performed at ARUP; separate specimens must be submitted when multiple tests are ordered. The MPL Mutation Analysis Test is a genetic test that detects abnormalities in the MPL gene. This assay will not detect the mutation below the sensitivity of this assay. Bearing in mind that thrombocytopenia can be the end result of pathogenetic mechanisms that are not mutually exclusive and that, particularly in the acute care setting, multiple potential causes of thrombocytopenia can be identified, a basic laboratory evaluation should include liver and renal function tests, a clotting screen with D-dimers, and measurement of lactate dehydrogenase.